Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody

Product Name

Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody

View all Thrombomodulin/BDCA-3 Antibodies

SKU/Catalog Number

M01325

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody (Catalog # M01325). Tested in ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Thrombomodulin / CD141 (Endothelial Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01325)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: THBD/1782

Isotype

IgG2a, kappa

Immunogen

Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01325 is reactive to THBD in Human

Applications

M01325 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.329kDa

Background of Thrombomodulin/BDCA-3

This monoclonal antibody recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-Thrombomodulin/CD141 Mouse Monoclonal Antibody (THBD/1782).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Anti-Thrombomodulin/CD141 Mouse Monoclonal Antibody (THBD/1782).

Gene/Protein Information For THBD (Source: Uniprot.org, NCBI)

Alternative Names

BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM THBD AHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM thrombomodulin thrombomodulin|CD141 antigen|fetomodulin

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